Dedifferentiation-derived neural stem cells exhibit perturbed temporal progression

K Veen; PK Nguyen; F Froldi; Q Dong; E Alvarez-Ochoa; KF Harvey; JPD McMullen; O Marshall; PR Jusuf; LY Cheng

Journal article

Dedifferentiation-derived neural stem cells exhibit perturbed temporal progression

K Veen, PK Nguyen, F Froldi, Q Dong, E Alvarez-Ochoa, KF Harvey, JPD McMullen, O Marshall, PR Jusuf, LY Cheng

EMBO Reports | Published : 2023

DOI: 10.15252/embr.202255837

Abstract

Dedifferentiation is the reversion of mature cells to a stem cell-like fate, whereby gene expression programs are altered and genes associated with multipotency are (re)expressed. Misexpression of multipotency factors and pathways causes the formation of ectopic neural stem cells (NSCs). Whether dedifferentiated NSCs faithfully produce the correct number and types of progeny, or undergo timely terminal differentiation, has not been assessed. Here, we show that ectopic NSCs induced via bHLH transcription factor Deadpan (Dpn) expression fail to undergo appropriate temporal progression by constantly expressing mid-temporal transcription factor(tTF), Sloppy-paired 1/2 (Slp). Consequently, this r..

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University of Melbourne Researchers

Joanna Dong Author

Kieran Harvey Author

Patricia Jusuf Author

Louise Cheng Author

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Grants

Awarded by University of Melbourne

Funding Acknowledgements

We are grateful to C. Desplan, H. Richardson, S.Russell, X Li, U.Walldorf, K Saito, for generous sharing of antibodies and fly stocks. LYC is funded by an Australian Research Council (ARC) Future Fellowship (FT180100255), LYC's laboratory is supported by funding from the National Health and Medical Research Council of Australia (NHMRC 1182847), Australian Research Council (190101743) and the Peter MacCallum Cancer Foundation. KFH was supported by an NHMRC Investigator grant (APP1194467) and Australian Research Council (190101743). Confocal Microscopy was performed at the Centre for Advanced Histology & Microscopy (CAHM) at the Peter MacCallum Cancer Centre and the Biological Optical Microscopy Platform at the University of Melbourne. We thank CAHM and Research Laboratory Support Services and acknowledge support to them by the Peter MacCallum Cancer Foundation and the Australian Cancer Research Foundation. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.